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Acinetobacteria are non-fermenting gram-negative coccobacilli that have been identified as one of the leading causes of nosocomial infections over the last three decades. The mortality rate from this bacterium is also relatively high, so that in a study conducted in 2010, out of 146 patients with Multi-drug resistant Acinetobacter pneumoniae (MDR), 23 (15.8) of them died. The cyclin is the first FDA-approved antibiotic for glycine glycine antibiotics.

This antibiotic has significant potency against gram-negative and gram-positive bacteria and anaerobic bacteria including multidrug-resistant strains (MDRs). The resistance of Acinetobacter baumannii to this antibiotic is not very high, but today we see a gradual increase in resistance to this antibiotic, which makes it difficult to treat these infections. One of the most important and widely used methods for molecular typing and clonal relationship evaluation of bacterial isolates is the Multilocus sequence typing (MLST) technique.

Given the above and the need to understand the mechanisms of resistance and profiles related to ticycline resistance, we decided to conduct a study entitled: Prevalence of ticycline-resistant strains in clinical isolates of Acinetobacter baumannii and evaluation of their genetic relationship using Perform MLST, MLVA and Multiplex PCR sequencing.

This method is one of the high sensitivity molecular methods and is used today to study the typing pattern in different bacteria. In recent years, with the introduction and development of molecular typing methods such as Variable number tandem repeat and Multiplex PCR, significant genetic diversity has been observed among Acinetobacter baumannii strains. In the Multiple loci VNTR analysis (MLVA) technique, by selecting different loci in terms of each repeating unit size and longitudinal polymorphism, the ability to differentiate between strains is provided.