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Ethics code: IR.MEDILAM.REC.1397.86
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abbasi N. Synthesis of Dendrimer (nanopolymer) and use as drug delivery silibinin from Silybum Marianum Assesment of Neurotrophic and angiogenic Effects of Silibinin on Olfactory Ensheathing Cells (OECs) of rat in Normal and high glucose conditions. Journal title 2024; 1403 (1)
URL: http://newresearch.medilam.ac.ir/article-1-2940-en.html
URL: http://newresearch.medilam.ac.ir/article-1-2940-en.html
biotechnology and medicinal plants
Abstract: (9 Views)
Drug delivery systems have been of interest to researchers. The effects of synthesized nano-polymers as
silibinin and silymarin extract drug delivery systems on olfactory ensheathing cells under normal and
high-glucose conditions were studied. The structure of the nanopolymer w:as char:acterized by IR,
HNMR, GPC, DLS, and AFM. The toxicity was evaluated by an MTT assay. The production of ROS and the
generation of NO were evaluated by a probe of fluorescein diacetate and Griess methods, respectively.
The expressions of the protein levels of ILK, VEGF, BDNF, and NGF were investigated by western blotting.
The polymer size was between 50 and 150 nm. The loading capacities for silibinin and silymarin were
68.5% and 56.4%, respectively, and the drug release for them was estimated at 54.1% and 50.8%,
respectively. In high-glucose conditions, the cells were protected (EC50 ¼ 4.88 0.5 mM) by silibinin and
nanopolymer in low concentrations by reducing the amount of ROS and NO, maintaining ILK, reducing
VEGF and increasing NGF and BDNF. Incubation with silibinin and nanopolymer at high concentrations
increased cell death with LC50 ¼ 57.36 2.5 and 43.18 1.8 mM, respectively, in high-glucose states.
Thus, the cells were protected by silibinin and nanopolymer in protective concentrations by reducing the
amount of ROS and NO, maintaining ILK, reducing VEGF, and increasing BDNF and NGF. The mentioned
mechanisms were totally reversed at high concentrations.
silibinin and silymarin extract drug delivery systems on olfactory ensheathing cells under normal and
high-glucose conditions were studied. The structure of the nanopolymer w:as char:acterized by IR,
HNMR, GPC, DLS, and AFM. The toxicity was evaluated by an MTT assay. The production of ROS and the
generation of NO were evaluated by a probe of fluorescein diacetate and Griess methods, respectively.
The expressions of the protein levels of ILK, VEGF, BDNF, and NGF were investigated by western blotting.
The polymer size was between 50 and 150 nm. The loading capacities for silibinin and silymarin were
68.5% and 56.4%, respectively, and the drug release for them was estimated at 54.1% and 50.8%,
respectively. In high-glucose conditions, the cells were protected (EC50 ¼ 4.88 0.5 mM) by silibinin and
nanopolymer in low concentrations by reducing the amount of ROS and NO, maintaining ILK, reducing
VEGF and increasing NGF and BDNF. Incubation with silibinin and nanopolymer at high concentrations
increased cell death with LC50 ¼ 57.36 2.5 and 43.18 1.8 mM, respectively, in high-glucose states.
Thus, the cells were protected by silibinin and nanopolymer in protective concentrations by reducing the
amount of ROS and NO, maintaining ILK, reducing VEGF, and increasing BDNF and NGF. The mentioned
mechanisms were totally reversed at high concentrations.
Subject:
General
Received: 2024/12/18 | Accepted: 2024/05/30 | Published: 2024/05/30
Received: 2024/12/18 | Accepted: 2024/05/30 | Published: 2024/05/30
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