fa بررسی مصرف همزمان امواج اولتراسوند و الکل در زمان جنینی بر میزان کلسیم و ROS داخل سلولی سلول های هیپوکامپ در زمان جنینی در موش صحرایی بافت و فیزیولوژی آزمایشگاهی Objective: Ultrasound (US) is an imaging procedure with various applications, such as checking the fetus during pregnancy. To the best of our knowledge no study have investigated the simultaneous effect of diagnostic US waves and ethanol exposure on intracellular calcium ([Ca2+]i) processes and reactive oxygen species (ROS). Thus, in the current study, we first aimed to investigate the impacts of diagnostic US during fetal life on [Ca2+]i and ROS in rats’ hippocampal cells; then whether the diagnostic US has intensified ethanol effects on these two variables. Materials and Methods: After mating, adult female Wistar rats were divided into six groups as follows: Control group, ethanol group (5 g/kg), groups that were exposed to diagnostic US at frequencies of 3 and 5 MHz for 10 minutes on the 11-13 days of pregnancy, and groups that underwent US waves accompanied with ethanol on the same term of pregnancy. Fourteen days after delivery, the offspring were anesthetized, the hippocampus was isolated, and [Ca2+]i was measured using the Flou 3-AM and fluorimetry instrument after cell culture. Results: The results showed that the diagnostic US with 3 and 5 MHz frequencies significantly increased [Ca2+]i levels up to 17.8% and 21.8 %, as well as ROS levels by 81.18% and 128.51% compared to the control group. In addition, [Ca2+]i levels in the groups with simultaneous exposure to ethanol and US with frequencies 3 and 5 MHz were 19.19% and 23.33% higher than the ethanol group. Besides, the amount of ROS in the groups with simultaneous exposure to ethanol and US with frequencies 3 and 5 MHz were 77.5% and 172.5% higher than the ethanol group, respectively. However, ethanol alone did not alter intracellular [Ca2+]i and ROS levels. Conclusion: It is concluded that diagnostic US with 3 and 5 MHz frequencies increase the [Ca2+]i and ROS rate, and it also intensifies the effects of ethanol on the [Ca2+]i and ROS levels. Keywords: Hippocampus, intracellular calcium, ultrasound, rat, ROS Objective: Ultrasound (US) is an imaging procedure with various applications, such as checking the fetus during pregnancy. To the best of our knowledge no study have investigated the simultaneous effect of diagnostic US waves and ethanol exposure on intracellular calcium ([Ca2+]i) processes and reactive oxygen species (ROS). Thus, in the current study, we first aimed to investigate the impacts of diagnostic US during fetal life on [Ca2+]i and ROS in rats’ hippocampal cells; then whether the diagnostic US has intensified ethanol effects on these two variables. Materials and Methods: After mating, adult female Wistar rats were divided into six groups as follows: Control group, ethanol group (5 g/kg), groups that were exposed to diagnostic US at frequencies of 3 and 5 MHz for 10 minutes on the 11-13 days of pregnancy, and groups that underwent US waves accompanied with ethanol on the same term of pregnancy. Fourteen days after delivery, the offspring were anesthetized, the hippocampus was isolated, and [Ca2+]i was measured using the Flou 3-AM and fluorimetry instrument after cell culture. Results: The results showed that the diagnostic US with 3 and 5 MHz frequencies significantly increased [Ca2+]i levels up to 17.8% and 21.8 %, as well as ROS levels by 81.18% and 128.51% compared to the control group. In addition, [Ca2+]i levels in the groups with simultaneous exposure to ethanol and US with frequencies 3 and 5 MHz were 19.19% and 23.33% higher than the ethanol group. Besides, the amount of ROS in the groups with simultaneous exposure to ethanol and US with frequencies 3 and 5 MHz were 77.5% and 172.5% higher than the ethanol group, respectively. However, ethanol alone did not alter intracellular [Ca2+]i and ROS levels. Conclusion: It is concluded that diagnostic US with 3 and 5 MHz frequencies increase the [Ca2+]i and ROS rate, and it also intensifies the effects of ethanol on the [Ca2+]i and ROS levels. Keywords: Hippocampus, intracellular calcium, ultrasound, rat, ROS Hippocampus, intracellular calcium, ultrasound, rat, ROS Hippocampus, intracellular calcium, ultrasound, rat, ROS 0 0 http://newresearch.medilam.ac.ir/browse.php?a_code=A-10-2598-3&slc_lang=fa&sid=1 Mohamadreza Kafashian محمدرضا کفاشیان m_r_kaffashian@yahoo.com 3329718927 100319475328460067787 Yes Department of Physiology, School of Medicine, Ilam University of Medical sciences, Ilam, Iran گروه آموزشی فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران Ardeshir Moayeri اردشیر معیری moayeri46@yahoo.com 2709278200 100319475328460067788 No Department of Anatomical Sciences, School of Medicine, Ilam University of Medical sciences, Ilam, Iran گروه آموزشی آناتومی و تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران Naser Abbasi ناصر عباسی ilamfarma@gmail.com 4559758026 100319475328460067789 No Biotechnology and Medicinal Plants Research Center, Ilam University of Medical sciences, Ilam, Iran مرکز تحقیقات زیست فناوری و گیاهان دارویی، دانشگاه علوم پزشکی ایلام، ایلام، ایران Maryam Maleki مریم ملکی maryammaleki777@yahoo.com 4500170065 100319475328460067790 No Department of Physiology, School of Medicine, Ilam University of Medical sciences, Ilam, Iran گروه آموزشی فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران elahe ranaei الهه رعنایی elaheranaeiana@gmail.com 4500763422 100319475328460067791 No Department of Physiology, School of Medicine, Ilam University of Medical sciences, Ilam, Iran گروه آموزشی فیزیولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی ایلام، ایلام، ایران mina kafashi مینا کفاشی kafashimina@gmail.com 4490134665 100319475328460067792 No