Objectives: The most common parasites responsible for fascioliasis are F. hepatica and F. gigantica. Fascioliasis is an important problem in terms of health and economics. The western part of Iran is one of the major hubs of animal husbandry and the prevalence of fascioliasis is high. The aim of
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Genotyping of Fasciola hepatica
Isolated from Domestic Animals
in the West of Iran
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11/30/22, 3:06 PM Genotyping of Fasciola hepatica Isolated from Domestic Animals in...: Ingenta Connect
https://www.ingentaconnect.com/content/ben/iddt/2019/00000019/00000004/art00011 2/2
the current study was to determine the Fasciola spp. present in the
western part of Iran. In the present study, 45 samples were collected from
slaughterhouses in three provinces in the western part of Iran, including
Ilam, Lorestan and Kermanshah.
Methods: The flukes were detected using morphological methods and the
DNA of all samples was extracted. The ribosomal internal transcribed
spacer (ITS1) was identified by PCR and PCR-RFLP techniques using the
Rsa1 restriction enzyme. Then, 15% of the samples were sequenced.
Results: Based on their ITS1 sequence, all samples showed 700 bp bands.
The results of sequencing showed a similarity of 99% to 100% across
samples. Using the Rsa1 restriction enzyme, all samples produced three
distinct bands (60 bp, 100 bp, and 360 bp). These results demonstrated
that the most common fasciola in the western part of Iran in sheep and
cattle is F. hepatica.
Conclusion: The results of the present study showed that only the species
F. hepatica is parasitizing livestock in the western part of Iran. Further
studies using new molecular markers for more accurate identification of
fasciola-causing species will be useful in the control and prevention of
fascioliasis.
 

Keywords: Fasciola, genotyping, Iran