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ghafurian S, abouali galedari E, norozi M, maleki A. Antitoxin Toxin System as an antimicrobial target in Clinical Acinetobacter baumannii. Journal title 2022; 5 (3)
URL: http://newresearch.medilam.ac.ir/article-1-2029-en.html
URL: http://newresearch.medilam.ac.ir/article-1-2029-en.html
Abstract: (462 Views)
Introduction:Acinetobacter baumannii is a gram-negative bacterium that is commonly associated with multi drug resistance of opportunistic infections. This bacterium can cause a wide range of infections including bacteremia, pneumonia, meningitis, urinary tract infections and wound infection in susceptible patients. Toxin-antitoxin (TA) system is a regulated system that could be used for new antimicrobial targets. Therefore, in this study, the antitoxin-toxin system was evaluated as an antimicrobial target in clinicalisolates of A.baumannii
Material and method: Eighty clinical isolates were collected from ICU and burn wards of Ilam hospitals during the year 2018 and were confirmed by biochemical and molecular methods of as Acinetobacter baumannii. Antibiotic resistance was performed by disfiguring disk on all isolates. The presence of MazE, MazF and HighA antitoxin toxin systems was performed by PCR method and expression of these systems by RT-PCR method.
Results: Resistance to antibiotics were detected for kanamycin (81.3%), tetracycline (48.8%), amikacin (51.3%), gentamycin (48.8%), tobramycin (28.8%), doxycycline (8.28%), and minocycline (7.5%). The frequency of toxin and antitoxin genes of MazE, MazF,HighA, and HighBin isolates was 62.5%, 46.3%, 38.8%, and 37.2% respectively. The frequency of plasmidTA systemesof MazE, MazF, HighA, and HighB in all isolates was 43.8%, 33.8%, 23.8%, and 21.0% respectively. The expression of the MazEF locus function was confirmed in all isolates by RT-PCR
Conclusion: Our findings indicate that there is a high antibiotic resistance in the Acinetobacter baumannii isolates. In this study, the high prevalence of the MazEF toxin-antitoxin system and the expression of this system in isolates has been proven. Therefore, the deactivation of the MazEF loci could be used as a new antimicrobial strategy in the treatment of resistant infections caused by this bacterium.
Material and method: Eighty clinical isolates were collected from ICU and burn wards of Ilam hospitals during the year 2018 and were confirmed by biochemical and molecular methods of as Acinetobacter baumannii. Antibiotic resistance was performed by disfiguring disk on all isolates. The presence of MazE, MazF and HighA antitoxin toxin systems was performed by PCR method and expression of these systems by RT-PCR method.
Results: Resistance to antibiotics were detected for kanamycin (81.3%), tetracycline (48.8%), amikacin (51.3%), gentamycin (48.8%), tobramycin (28.8%), doxycycline (8.28%), and minocycline (7.5%). The frequency of toxin and antitoxin genes of MazE, MazF,HighA, and HighBin isolates was 62.5%, 46.3%, 38.8%, and 37.2% respectively. The frequency of plasmidTA systemesof MazE, MazF, HighA, and HighB in all isolates was 43.8%, 33.8%, 23.8%, and 21.0% respectively. The expression of the MazEF locus function was confirmed in all isolates by RT-PCR
Conclusion: Our findings indicate that there is a high antibiotic resistance in the Acinetobacter baumannii isolates. In this study, the high prevalence of the MazEF toxin-antitoxin system and the expression of this system in isolates has been proven. Therefore, the deactivation of the MazEF loci could be used as a new antimicrobial strategy in the treatment of resistant infections caused by this bacterium.
Received: 2015/10/3 | Accepted: 2016/03/9 | Published: 2016/12/30
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