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Mirzaei A, alikhani M, Pakzad I. Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation in patients refferd to Ilam hospitals. Journal title 2022; 5 (1)
URL: http://newresearch.medilam.ac.ir/article-1-1888-en.html
URL: http://newresearch.medilam.ac.ir/article-1-1888-en.html
Zoonotic Diseases Research Center, Ilam University of Medical sciences, Ilam, Iran
Abstract: (1311 Views)
Background: Trichomoniasis is one of the parasitic diseases caused by the flagellate protozoan Trichomonas vaginalis. In this regard, there is not any study for the genetic diversity of T. vaginalis in Ilam County. Therefore, the current research is aimed to recognize Genetic variability between Trichomonas vaginalis isolates and correlation with clinical presentation in patients refferd to Ilam hospitals.
Material and methods: The current study was performed among 503 women referred to Gynecology clinics in the Ilam County. Sterile cotton swabs from the posterior vaginal fornix and urine specimen were collected from women who referred to the Gynecology clinics of Ilam County. Smears were stained by Giemsa and positive sample subjected to PCR techniques for genetic diversity.
Results:
The finding of this study revealed no significant associations between trichomonas infection with age, prevention of pregnancy, abortion, stillbirth or preterm delivery (p> 0.05). On the other hand, there was a significant associations between trichomonas infection and history of previous diseases in the past year (p <0.05). Totally, 20 (3.9 %) were positive for T. vaginalis. In this study, the infection was more common in women aged between 25-25 years. The current isolates showed high homology and alignment (97- 100%) with reference sequence of T. vaginalis in the GenBank. There was no genetic diversity among T. vaginalis isolates, which it could be due to geographic limitation at sampling time.
Conclusion: To our knowledge, this is the first description of genetic diversity of T. vaginalis in Ilam County. Moreover, ITS marker useful tool for identification of Trichomonas vaginalis and genetic diversity.
Material and methods: The current study was performed among 503 women referred to Gynecology clinics in the Ilam County. Sterile cotton swabs from the posterior vaginal fornix and urine specimen were collected from women who referred to the Gynecology clinics of Ilam County. Smears were stained by Giemsa and positive sample subjected to PCR techniques for genetic diversity.
Results:
The finding of this study revealed no significant associations between trichomonas infection with age, prevention of pregnancy, abortion, stillbirth or preterm delivery (p> 0.05). On the other hand, there was a significant associations between trichomonas infection and history of previous diseases in the past year (p <0.05). Totally, 20 (3.9 %) were positive for T. vaginalis. In this study, the infection was more common in women aged between 25-25 years. The current isolates showed high homology and alignment (97- 100%) with reference sequence of T. vaginalis in the GenBank. There was no genetic diversity among T. vaginalis isolates, which it could be due to geographic limitation at sampling time.
Conclusion: To our knowledge, this is the first description of genetic diversity of T. vaginalis in Ilam County. Moreover, ITS marker useful tool for identification of Trichomonas vaginalis and genetic diversity.
: Cross sectional |
Received: 2022/05/14 | Accepted: 2022/06/19 | Published: 2022/06/19
Received: 2022/05/14 | Accepted: 2022/06/19 | Published: 2022/06/19
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