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Sadeghifard N, teimourpour M. Genetic Analysis of Multiple Drug-resistant Klebsiella pneumoniae Isolatesby Pulsed-field Gel Electrophoresis. Journal title 2021; 4 (3)
URL: http://newresearch.medilam.ac.ir/article-1-1649-en.html

Genetic Analysis of Multiple Drug-resistant Klebsiella pneumoniae Isolatesby Pulsed-field Gel Electrophoresis

Nourkhoda Sadeghifard

, Maria Teimourpour

Clinical Microbiology Research Center, Ilam University of Medical sciences, Ilam, Iran

Abstract: (1797 Views)

Hospitals such as pneumonia, urinary tract infections, septicemia, bacterial meningitis and biliary tract infections. Klebsiella is a major cause of nosocomial infections, especially among neonatal and intensive care unit patients with high mortality rates over the past two decades. The incidence of infections caused by multidrug-resistant Klebsiella species has increased. Multidrug resistance in Klebsiella specimens is associated with health care, using only carbapenems as the last possible line of antibiotic defense for infection. Unfortunately, since the early 2000s, Klebsiella strains of multidrug-resistant specimens (MDRs) have begun to produce carbapenemases encoded by plasmids. Which quickly spread to outpatients and hospitalized patients. There are methods for detecting the occurrence of an epidemic from related and unrelated strains as well as comparing specimens at the subspecies level, the most important of which is the epidemiological typing method that brings us closer to this goal. These methods rely on enzymatic cleavage and typing based on PCR and PFGE, which explore the molecular epidemiology of Klebsiella's sample and facilitate cognitive facilitation, as well as isolated strains to better understand the epidemiology of MDR strains. PFGE is often considered the gold standard of other methods, which is a reference method for typing and identifying many organisms, including Klebsiella. The aim of this study was to evaluate the pattern of antibiotic susceptibility as well as PFGE in order to study the molecular epidemiology of Klebsiella strains isolated from hospital clinical patients. Given the importance of MDR in bacteria, the need for new programs to prevent their spread is evident. The misguided policy of overuse of antibiotics, resource constraints, and lack of facilities in hospitals has contributed to these conditions, which have limited the effectiveness of many antimicrobial agents, resulting in significantly reduced treatment choices, which is why Doctors go straight to strong antibiotics, and prescribe the strongest antibiotics. The need for further studies in this field, as well as reducing the use of antibiotics and prescribing antibiotics by physicians is essential due to the results of antibiotic susceptibility testing. In this study, we tried to study the genetic analysis of Klebsiella isolates with multiple drug resistance and antibiotic resistance pattern and determine their types, because to prevent and control infections and to select appropriate treatment, genetic analysis and epidemiological study of these isolates are required. . In this study, by recognizing the clonal relationship of Klebsiella sample isolates and examining their genetic diversity, it can be useful and practical in identifying the source and controlling infections caused by Klebsiella sample.

Keywords: Genetic Analysis - Multiple Drug-resistant - Klebsiella pneumoniae - Pulsed-field- Gel Electrophoresis

: Cross sectional |
Received: 2021/10/13 | Accepted: 2021/12/1 | Published: 2021/12/1

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