Volume 2, Issue 4 (2-2020)
2020, 2(4): 0-0 |
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Ethics code: IR.MEDILAM.REC.1397.150
: Isolation and determine genotype of Acanthamoeba from produce soil of haze in Ilam and Mehran city
Zoonotic Diseases Research Center, Ilam University of Medical sciences, Ilam, Iran
Abstract: (1783 Views)
In recent years, increasing dust phenomenon in the west of Iran has led to
social, economic, and health concerns. This study aimed to represent the existence and
genotyping of Acanthamoeba spp. in dust phenomenon in Ilam Province, Iran. In
this study, 55 dust samples were collected and by targeting the diagnostic fragment
3 region of the 18S rRNA gene, the genotypes were determined. Utilizing the tolerance
ability test, the pathogenic potential of all positive isolates was also recognized.
Eighteen samples of Acanthamoeba (32.7%) were detected in the sampling areas.
According to sequencing analysis, the isolates related to T4 (77.7%) and T2 (22.3%)
genotypes were reported. It was revealed by thermo- and osmotolerance tests in
which six strains are extremely pathogenic. To our knowledge, the pathogenic
Acanthamoeba was potentially isolated initially from dust phenomenon in Ilam
Province. Thus, these strains are probably highly virulent, and dusts are possible
sources of Acanthamoeba infection in humans
social, economic, and health concerns. This study aimed to represent the existence and
genotyping of Acanthamoeba spp. in dust phenomenon in Ilam Province, Iran. In
this study, 55 dust samples were collected and by targeting the diagnostic fragment
3 region of the 18S rRNA gene, the genotypes were determined. Utilizing the tolerance
ability test, the pathogenic potential of all positive isolates was also recognized.
Eighteen samples of Acanthamoeba (32.7%) were detected in the sampling areas.
According to sequencing analysis, the isolates related to T4 (77.7%) and T2 (22.3%)
genotypes were reported. It was revealed by thermo- and osmotolerance tests in
which six strains are extremely pathogenic. To our knowledge, the pathogenic
Acanthamoeba was potentially isolated initially from dust phenomenon in Ilam
Province. Thus, these strains are probably highly virulent, and dusts are possible
sources of Acanthamoeba infection in humans
: Cross sectional |
Subject:
General
Received: 2018/12/22 | Accepted: 2019/01/28 | Published: 2020/02/29
Received: 2018/12/22 | Accepted: 2019/01/28 | Published: 2020/02/29
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