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Molecular identification of Entamoeba histolytica from stool samples of Ilam
Razi Naserifar Adviser * , Azar Najafi Student, Jahangir Abdi Consultant , Ali Kermanjani Consultant
Zoonotic Diseases Research Center, Ilam University of Medical sciences, Ilam, Iran
Abstract:   (374 Views)
Introduction:
Amoebiasis is a multifactorial, life-threatening public health issue and presumably the third parasitic disease cause of mortality - world wide,The aim of this study identified  of  E. histolytica in  from stool samples isolated from Central laboratory and in Imam -Khomeini hospital at the University of Medical Sciences ,and Ilam city which is located in southwest Iran, from 2017 to 2018.
Materials and Methods:
Totally,2023 samples were examined with microscopy, and Thirty-duplicated Entamoeba histolytica/ dispar isolates were collected from hospital in Ilam, Iran. The isolates were identified and the polymerase chain reaction (PCR) was performed to detect the CP8 gene.
Results: In genotype confirmation,  only were two isolates - the CP8  gene found in the PCR test. The sequencing results confirmed the afro-mentioned gene with 97% specifity.
Conclusion: It is concluded that PCR are highly sensitive to detect Entamoeba histolytica  and play a fundamental  role as screening tools in direct DNA extraction from stool samples in early detection of symptomatic and asymptomatic E. histolytica patients.
Keywords: Entamoeba histolytica, Identification, PCR, western Iran
     
: Cross sectional | Subject: General
Received: 2017/10/23 | Accepted: 2017/12/9 | Published: 2020/03/15
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Ethics code: IR.MEDILAM.REC.1397.150


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Naserifar R, Najafi A, Abdi J, Kermanjani A. Molecular identification of Entamoeba histolytica from stool samples of Ilam. 3. 2020; 2 (4)
URL: http://newresearch.medilam.ac.ir/article-1-726-en.html


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